With the relative ease of collection, expression profiling of human blood has an increasingly important role in both drug development and the identification of surrogate biomarkers of disease. But RNA isolated from whole blood specimens is derived from a mixture of cell types and contains a high proportion of transcripts encoding globin polypeptides, which have a tendency to reduce the sensitivity of microarray hybridization results.
To resolve this challenge, Expression Analysis pretreats globin-rich RNA specimens with peptide nucleic acid oligomers, which interfere with globin RNA transcription. This Globin RNA Reduction procedure leads to increased transcript detection and reduced signal variation across replicates on some microarray platforms.
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EXPRESSION PROFILING AND GLOBIN RNA REDUCTION
In order to increase the number of transcripts detected in GeneChip® assays, we recommend that the whole blood RNA samples be treated with a globin RNA reduction procedure before beginning target generation.
Click here to read the technical note, Globin RNA Reduction in Blood Samples and Affymetrix GeneChips
Click here to read the technical note, Expression Profiling with Blood Specimens and Illumina BeadChips
RNA AND DNA ISOLATIONS FROM PAXGENE AND TEMPUS TUBES
Expression Analysis specializes in the purification of RNA from whole blood using either PAXgene™ Blood RNA tube or Tempus™ Blood RNA Tubes . These products offer an integrated and standardized system for the collection and stabilization of whole blood specimens followed by the isolation of cellular RNA. Benefits include immediate stabilization of both the RNA and the expression profile. The PAXgene product has received 510(k) approval by the FDA for use in clinical and diagnostic applications. The Tempus system is for research use only, and not for use in diagnostic procedures.
GENOTYPING USING DNA FROM WHOLE BLOOD SPECIMENS
Expression Analysis has successfully used DNA isolated from blood specimens in several genotyping studies.
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