Illumina Products & Services from Expression Analysis
SERVICES NOW AVAILABLE
 Illumina BioArray® technology is based on optically wired 3-micron beads that self assemble in micro-wells etched on a silica slide, referred to as a BeadChip. Each bead is covered with a specific oligonucleotide, often a 50-mer, and multiple copies of each bead type are present on one microarray. Illumina products are designed for high-throughput testing of multiple specimens in parallel. BeadChips contain up to 12 microarrays per slide and the Illumina Array Matrix format attaches beads to fiber optic bundles that are compatible with 96-well plates.
Expression Analysis supports the following applications of Illumina products:
Expression Profiling using Whole Genome BeadChips
Quantify 48,000 transcripts with 6 microarrays per slide or 22,000 well-characterized genes selected from the NCBI RefSeq databases with 8 or 12 microarrays per slide
Human-6, v2 BeadChip, Human Ref8 v2 BeadChip,
Mouse-6, v2 BeadChip, Mouse Ref8 v2 BeadChip and Rat Ref-12 BeadChip
Whole Genome Genotyping and Copy Number Analyses using Infinium® Assays
Identify up to 655,000 tag SNP markers using the Infinium® II assay, which combines whole genome amplification, sequence capture by hybridization and allele-specific base extension
Human Hap650Y BeadChip, Human Hap550 BeadChip and
Human 1M BeadChip with >1 million SNPs including mitochondrial, ADME, MHC and CNV regions
Focused Genotyping using GoldenGate® Assays
Identify 384 to 1,536 focused SNPs
with the GoldenGate® assay, which amplifies extension-ligation products using pre-developed or customized panels of allele-specific primers
Human Cancer Panel with 1,400 SNPs derived from more than 400 genes
Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues using DASL® Assays
Quantify focused sets of transcripts in partially degraded RNA samples using the DASL® assay, which includes random primers during cDNA synthesis and amplifies extension-ligation products using pre-developed or customized panels of gene-specific primers
DASL® Human Cancer Panel with 3 probes each for 502 genes
MicroRNA Expression Profiling
Illumina has developed a powerful method for profiling the expression levels of a wide range of miRNA based ontheir proven DASL assay. These miRNA expression multiplexed assays have been designed to measure the levels of 735 or 380 miRNA in human or mouse samples, respectively, and are deployed on a 96-sample array matrix.
Additional BioArray® applications are available upon request.
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