ADOPT RNA SEQ ASAP TO MOVE YOUR RESEARCH IN THE RIGHT DIRECTION.

Overview:

mRNA-Seq (also known as transcriptome sequencing) potentially allows profiling the entire population of mRNA species and potentially enables mapping and quantification of all transcripts. It can also provide information on alternative splice sites, novel transcripts, previously unknown isoforms, rare transcripts and cSNPs.

Purpose:

Transcriptome studies are a cornerstone of functional genomics and provide insight into which genes are expressed in specific circumstances, such as tissue-specific expression patterns, organismal and strain variation in expression patterns and differential expression in disease conditions. In contrast to microarray-based analyses, mRNA-Seq does not rely upon a predetermined set of probe sequences.

Highlights:

• mRNA-Seq is a universal discovery platform used to sequence eukaryotic mRNA without the need for prior sequence information.
• mRNA-Seq is flexible that allows deep sequencing for detecting rare events or basic sequencing to be comparable or better overall than microarray-based results.

Advantages:

• More versatile and comprehensive than microarrays, with superior reproducibility.
• Enables comparisons between transcript isoforms.
• Open platform that does not depend on any prior sequence information.
• Provides information about potentially all mRNA’s within the sample and allows for the discovery of novel mRNA’s.
• High sensitivity facilitates the detection of low abundance mRNAs.

Assay Summary:

mRNA-Seq is a cDNA sequencing application that generates a more comprehensive, quantitative view of the mRNA portion of the transcriptome. With no probes or primer design needed, RNA-Seq has the potential to provide relatively unbiased sequence information from polyA-tailed RNA for analysis of gene expression, novel transcripts, novel isoforms, alternative splice sites, allele-specific expression, cSNPs, and rare transcripts in a single experiment, depending on read depth.

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