Formalin-Fixed Paraffin-Embedded Samples (FFPE)*
SERVICES NOW AVAILABLE
The fixation of human tissues with formalin, and their subsequent embedding in paraffin, has been a routine method of preserving surgical samples for many years. But these extensive resources are rarely used in microarray-based gene expression or chromosome alteration studies, because nucleic acids isolated from FFPE sources are typically degraded.
Expression Analysis has partnered with NuGEN Technologies to optimize their WT-Ovation™ FFPE System. This
whole transcriptome amplification solution uses NuGEN’s proprietary Ribo-SPIA™ technology to generate biotin-labeled hybridization targets from small and degraded RNA samples that perform well in microarray expression profiling studies.
 Read Dr. Tom Goralski's description in Affymetrix User Forum, Profiling Services for FFPE Samples
Read interview in Affymetrix User Forum, Gianfranco de Feo of NuGEN Technologies
Read press release, Expression Analysis is First Provider of NuGEN's Solution for FFPE Specimens
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EXPRESSION PROFILING USING RNA ISOLATED FROM FFPE TISSUES
RNA samples from FFPE specimens are amplified and labeled using the WT-Ovation
™ FFPE System. Depending on the tissue procurement, fixation method and length of FFPE storage, a high percentage of transcripts, up to 50%, can be detected.
Click here for the technical note, Expression Profiling in FFPE Specimens on Affymetrix GeneChips
Click here for the technical note, Expression Profiling in FFPE Specimens on Illumina BeadChips
RNA AND DNA ISOLATION FROM FFPE TISSUES
Expression Analysis uses the FormaPure Isolation Kit from Agencourt Biosciences to simultaneously isolate RNA and DNA from 10-micron sections of FFPE tissue. Although the nucleic acids are often degraded, they can be used in microarray and QRT-PCR assays.
Additional services are also in development:
Genotyping & Copy Number Analyses using DNA from FFPE tissue
Quality Control Metrics for the Prediction of Hybridization Performance
Please register below if you would like to be alerted when additional FFPE services are available.
Click here to register for FFPE Alerts!
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