SERVICES

Expression Analysis offers both discovery and clinical genotyping services using four genomic platforms to identify single nucleotide polymorphisms (SNPs) and other genetic markers.

High-Density Genotyping – Microarrays with a million markers for Whole Genome Association Studies

Medium-Density Genotyping – Targeted assays for 384 to 20,000 SNPs using focused content arrays

Low-Density Genotyping  –  Services for individual loci or small sets of SNPs

Expression Analysis performs other DNA analysis services using microarrays.

Copy Number and LOH Analysis – DNA amplifications and deletions related to cancer and other diseases

Resequencing – Microarrays for sequencing both strands of up to 300,000 bases

Data deliverables include raw data files as well as Microsoft Excel summaries of SNP and sample performance.

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GENOTYPING SERVICES USING WHOLE-GENOME MICROARRAYS

Expression Analysis offers whole-genome genotyping services on the following platforms:

		Illumina high-density DNA Analysis products screen approximately 300,000 to over one million human SNPs per microarray.  The tagSNP probes serve as proxies for neighboring loci.  The accompanying Infinium II assay includes a whole-genome amplification method and an enzymatic discrimination step. Click here for more information »
		the HeliScope™ Single Molecule Sequencer has been designed to enable affordable and rapid resequencing of whole human genomes. This provides a direct and comprehensive catalog of genomic mutations.
		Affymetrix genome-wide GeneChip Mapping arrays screen approximately 10,000 to one million human SNPs per microarray.  Each SNP is interrogated by a set of different allele-specific probes.  The Human SNP assay kit amplifies known polymorphic regions in genomic DNA after enzyme digestion. Click here for more information »

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GENOTYPING SERVICES FOR INDIVIDUAL LOCI

Expression Analysis offers low-density genotyping services on two platforms.

		Applied Biosystems has designed thousands of human and mouse TaqMan® SNP Genotyping assays, which utilize TaqMan® chemistry to amplify and discriminate alleles for a specific SNP, including many in drug metabolism genes. Click here for more information »

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TARGETED GENOTYPING USING FOCUSED CONTENT MICROARRAYS

Expression Analysis offers medium-density genotyping services on two platforms.

		Illumina focused content DNA Analysis products screen panels of 384 to 1,536 SNPs in high throughput formats, such as 16-sample BeadChips or 96-sample matrixes.  Larger panels are also available.  The GoldenGate assay includes a single base chain extension for allele discrimination. Click here for more information »
		The Affymetrix Targeted Genotyping System screens approximately 3,000 to 20,000 SNPs on application-specific or custom microarrays.  These assays incorporate Molecular Inversion Probe technology, which generates allele-specific targets labeled with one of four unique fluorescent dyes. Click here for more information »

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COPY NUMBER AND LOH ANALYSES

Expression Analysis offers a variety of analysis options for estimating chromosomal copy number changes or identifying Loss of Heterozygousity (LOH) regions. These analyses rely on data from the polymorphic and nonpolymorphic markers on the Illumina and Affymetrix whole-genome microarrays.  Results are returned in tabular and/or graphical displays, including an aligned chromosomal view.

Click here for more information »

RESEQUENCING

Expression Analysis supports custom Affymetrix CustomSeq® Resequencing GeneChips® which deliver 300,000 bases of double-stranded sequence per microarray.  Sequences may cover a single contiguous region or multiple dispersed fragments, facilitating the analysis of whole genomes, multiple genes, and/or multiple organisms on a single array.  Expression Analysis has also processed Human Mitochondrial Resequencing Arrays.

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