University of Arizona
Expression Analysis is utilizing the services of the University of Arizona’s Genetics core to offer 454 sequencing.
The 454 Genome Sequencer FLX Instrument, powered by GS FLX Titanium chemistry, features a groundbreaking combination of long reads (300-500bp), exceptional accuracy, and high throughput. The instrument uses a system based on 454’s sequencing-by-synthesis, technology.
Applications
The high throughput 454/GS FLX Titanium sequencing technology has many applications. Some of the most common are:
- Whole genome sequencing (de novo, resequencing) via:
- Amplicon sequencing - newly released with Titanium chemistry!
- Small RNA sequencing, including expressed sequence tags (ESTs)
- Transcriptome analysis
- 3kb, 8kb and 20kb Paired End Library Preparation and Sequencing
- Shotgun sequencing (bacteria, viruses) and
- BAC-based shotgun sequencing (animals, plants).
- Assembled de novo for novel organisms or
- Mapped to genome or transcriptome references for characterized organisms
View University of Arizona site