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Expression
Analysis has developed the first Proficiency Testing
program for microarray assays. In 2004, the program
performed three rounds of testing using rat samples.
In 2007, the program initiated testing using
human RNA samples developed as part of the Microarray
Quality Control (MAQC) project initiated by
the FDA.
 Click here for the product description, Human Proficiency Testing Program
During each round of testing, Expression Analysis
distributes identical RNA samples to multiple
laboratories. Each laboratory processes the samples
using their unique protocols and returns CEL files
to Expression Analysis for detailed review. The
data sets are analyzed using a number of quality,
sensitivity, reproducibility and comparability
metrics. Initial findings indicate that similar
biological results can be identified in replicate
microarray data sets generated in different labs
or in the same lab at different times.
These results are proving to be highly encouraging
as microarrays transition toward clinical diagnostic
applications, which will require repeated proficiency
tests. They also help establish quality review
procedures that assist in establishing microarray
standards and may be required when submitting
data for regulatory review.
Reference Documents
Poster on Monitoring
Performance Levels in Microarray Core Facilities
Publication
on the Value of Proficiency Testing
Poster
on Proficiency Testing Program with Rat Samples
Poster
on initial Data Comparability Studies
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