RNA - Total
Whole Genome
Whole genome hybridization-based arrays offer an economical method to quickly perform expression profiling of a few or a large set of specimens. Illumina expression arrays feature up-to-date content largely derived from a recent release of the NCBI RefSeq database. The Direct Hybridization format offers the highest multiplexing for whole-genome expression profiling of up to 48,000 transcripts. Data quality and reproducibility are supported in part by the high level of bead type redundancy (up to an average of 30 beads per probe) on every array. Array probes consist of a single 50-mer gene-specific probe for each transcript from the 3’ end of the transcript. A minimal amount of total RNA (50-100 ng) is required for the single-cycle in vitro transcription (IVT) reaction.
HumanHT-12 Expression BeadChip
HumanRef-8 Expression BeadChip
MouseWG-6 Expression BeadChip
Performance Specs
MouseRef-8 Expression BeadChip
Performance Specs
RatRef-12 Expression BeadChip
Performance Specs
Digital Gene Expression (DGE): Tag Profiling
Illumina offers a whole genome expression platform that can generate expression profiles for any transcript from any gene in any organism. DGE creates genome wide expression profiles through sequencing, not hybridization. This allows for the ability to identify, quantify, and annotate expressed transcripts on the level of the whole genome in the absence of existing microarrays or prior sequence knowledge. This assay offers unlimited dynamic range, a tunable depth of coverage for rare transcript discovery and quantification. DGE records the numerical frequency of a sequence in the library population, thereby eliminating background signals.
Digital Gene Expression: Tag Profiling
mRNA-Seq
Whole transcriptome mRNA-Seq supports both genome-wide expression discovery and profiling, enabling the discovery and measurement of transcript abundance over five orders of magnitude, and accurately quantify tissue-specific isoforms. This enables the generation of full sequence date from any poly-A tailed RNA to analyze novel transcripts, novel isoforms, alternative splice sites, rare transcripts, and cSNPs in one experiment.
Transcriptome Analysis: mRNA-Seq
Performance Specs