DNA - Whole Genome
De Novo Sequencing
The unique combination of read length, read depth, and flexible paired-end insert sizes makes Genome Analyzer data ideal for de novo sequencing. The system’s unparalleled raw read accuracy enables confident and efficient production of high quality, long contig assemblies. With the ability to generate up to 18 Gb of 75+ bp paired-end data per run, any lab can rapidly and cost-effectively de novo sequence any sized genome.
Numerous novel algorithmic approaches have been developed that make optimal use of the benefits of Illumina sequencing technology for de novo assembly. Using these tools, researchers are demonstrating the utility of Genome Analyzer data to assemble small and large, complex genomes.
- Paired Reads - Generate long scaffolds and highly accurate contigs using multiple insert lengths with high library diversity.
- Read Length - Use paired-end reads in excess of 2 x 100 bases for mammalian-scale de novo assembly.
- Raw-Read Accuracy - Access the highest yield of perfect reads and Q35 data to generate long, error-free contigs.
- Assembly Tools - Leverage a rapidly growing number of assemblers optimized for Genome Analyzer reads, such as Velvet, EULER-SR, and SSAKE.